Swp5, a spore wall protein, interacts with polar tube proteins in the parasitic microsporidian nosema bombycis zhi li,a,b guoqing pan,a tian li,a wei huang,a jie chen,a lina geng,a donglin yang,a linling wang,b and zeyang zhoua,b. Nosema bombycis is an obligate intracellular parasitic fungus that utilizes a distinctive mechanism to infect bombyx mori. A lack of appropriate labeling methods has limited the research of the cell cycle and protein locations in intracellular stages. Pebrine is a chronic and disastrous disease of the silkworm bombyx mori l. Identification and subcellular localisation of hexokinase2 in nosema bombycis. Genomewide transcriptional response of silkworm bombyx mori. Prevalence of nosema bombycis in malaysian field populations. Jiancheng sun 1, feng zhu 2,4, hongli chen 1, mingshuai yao 1, guanyu zhu 1. As one of the core framework proteins of nuclear pore complex npc, nucleoporin nupl70 acts as a structural adapter between the nucleolus and nuclear pore membrane and maintains the stability of npc structure through interaction with other proteins. Nosema bombycis nageli causes the pebrine disease of silkworms. In many fungi, ty3gypsy elements are more abundant than the ty1copia elements. Indirect immunofluorescence assays and recombinant hk overexpressed in bmn cells showed that nbhk localized in the nucleus and cytoplasm of host cell during the meront stage. Microsporidia are a group of obligate intracellular parasites of medical and agricultural importance, which can infect almost all animals, including human beings. Engineered resistance to nosema bombycis by in vitro expression of.
Pdf the role of nbtmp1, a surface protein of sporoplasm. Biochemical characterization of the microsporidian nosema. The sporoplasm is the initial stage of microsporidian infection and proliferation, but its morphological and molecular characteristics are poorly understood. On the function of the polar filament of nosema bombycis volume 29 issue 2. Preliminary genomic characterization of microsporidian nosema. Molecular analysis of the alphatubulin gene from the. Nosema bombycis is a pathogen of the silkworm that belongs to the microsporidia, a group of obligate intracellular parasites related to fungi. Major microsporidian structural proteins, such as the spore wall protein swp, are known to be involved in host inv. In this study, a singlechain variable fragment scfv dependent technology is developed for the purpose of inhibiting parasite proliferation in insect cells. The microsporidian lifecycle includes a dormant extracellular phase and intracellular. A secretory hexokinase plays an active role in the. Rapid and sensitive detection of nosema bombycis using loopmediated isothermal ampli.
Genomewide transcriptional response of silkworm bombyx. In this study, the sporoplasm of nosema bombycis was successfully isolated and characterized after the induction of spore. Nosema bombycis suppresses host hemolymph melanization. Morphology and transcriptome analysis of nosema bombycis sporoplasm and insights into the initial infection of microsporidia qiang he, a,bjian luo, jinzhi xu, a,bchunxia wang, xianzhi meng, guoqing pan, tian li, zeyang zhoua,b,c. Pa, nosema antheraeae and silkworm p50 strain of bombyx mori were maintained in the sericulture research institute at the chinese academy of agricultural sciences. Preliminary genomic characterization of microsporidian. So far, no effective treatment could be found for pebrine. Mar 16, 20 background microsporidian nosema bombycis has received much attention because the pebrine disease of domesticated silkworms results in great economic losses in the silkworm industry. Pesquisas dos corpusculos nosema bombycis em diapausa. The protein profile of partially solubilized spores showed three major peptide bands of molecular weight 68, 94 and 100 kda.
However, this strategy is not efficient and provides no effective method to screen and enrich the. In this study, we identified a nup170 protein in the microsporidian nosema bombycis for the first time and named it as nbnup170. Easy labeling of proliferative phase and sporogonic phase of. Hostparasite relationship between cynaeus angustus lec. It is caused by nosema bombycis belonging to family nosematidae of the order. Aug 24, 2018 nosema bombycis is a sporeforming parasite causing microsporidiosis in silkworm bombyx mori. Methionine aminopeptidase 2 metap2, an essential gene of n. Zhenjiang, nosema bombycis, nosema philosamiae, nosema sp.
Nosema bombycis causes silkworm pebrine and significant economic losses for the silk industry due to horizontal transmission and vertical transmission. Microsporidia it is a unique pathogen transmitted through egg. Nosema bombycis is the first described microsporidium naegli, reference naegli 1857 recognized as the causative agent of silkworm pebrine which prevailed in europe, america and asia during the mid19th century becnel and andreadis, reference becnel, andreadis, wittner and weiss 1999 and is still epizootic and causing heavy economic losses. Sep 21, 2016 the microsporidian nosema bombycis is an obligate intracellular pathogen of the silkworm bombyx mori, causing the epidemic disease pebrine and extensive economic losses in sericulture. Molecular and biochemical responses in the midgut of the silkworm. The molecular data indicate the necessity of future taxonomic reevaluation of the genera nosema and vairimorpha. Because of its long incubation period and horizontal and vertical transmission, it is subject to quarantine measures in sericulture production. Within the fungi, retrotransposons are widespread and abundant table 1.
Improving phylogenetic inference of microsporidian nosema. Most parasitise insects and other arthropods, and the bestknown nosema species parasitise honeybees, where they are considered a significant disease by beekeepers, often causing a colony to fail to thrive in the spring as they come out of their. Characterization of active ribosomal rna harboring mites. In this report, an easy fluorescent labeling method has been developed to mark the proliferative and sporogonic phases of microsporidia nosema bombycis in host cells. Tommaso landolf 19081979, writer and translator graduated or literature at the. Nosema bombycis and nosema apis is not of cellulose natur c tests for chitin. The microsporidian nosema bombycis is an obligate intracellular parasite of bombyx mori, that lost its intact tricarboxylic acid cycle and mitochondria during evolution but retained its intact glycolysis pathway. A monoclonal antibody that tracks endospore formation in the. It is recognized as the pathogen of silkworm pebrine and still causes heavy economic losses in silkproducing countries such as china 5. Jan 25, 2021 background nosema bombycis is a unicellular eukaryotic pathogen of the silkworm, bombyx mori, and is an economic and occupational hazard in the silkworm industry. The interaction between spore wall proteins swps and polar tube proteins ptps in the formation, arrangement, orderly orientation, and function of the polar tube and spore wall remains to be determined. Nosema bombycis, polar filament, primary spore, environmental spore, gender specificity introduction pebrine is a deadly disease of the mulberry through the eggs i.
Ristorante sorrento ristoranti in penisola sorrentina, il tuo ristorante. Nosema bombi, a microsporidian pathogen of the bumble bee. Detailed understanding of the host bombyx mori response to infection by n. Identification of a nosema bombycis microsporidia spore. A monoclonal antibody that tracks endospore formation in. Molecular characterization of nosema bombycis methionine. Nosema bombycis is the first described microsporidium, which prevailed in europe, america, and asia during the mid19th century. Microsporidiosis of silkworm, bombyx mori lepidoptera. Swp5, a spore wall protein, interacts with polar tube. However, pebrine disease in silkworms has been bombycidae caused by the parasite, nosema kept. Rapid and sensitive detection of nosema bombycis using loop.
The iodinophilous vacuole 70 summary 73 bibliography. Swp5, a spore wall protein, interacts with polar tube proteins in the parasitic microsporidian nosema bombycis zhi li,a,b guoqing pan,a tian li,a wei huang,a jie chen. Comparing the genomic regions that harbor the respective subunit genes between n. Nosema bombycis, the first reported microsporidium, is a type of obligate intracellular parasite that infects silkworms and can be induced to germinate in alkaline solution in vitro. The genomic survey of tc1like elements in the silkworm. In vitro cultivation of microsporidia of clinical importance. Nosema bombycis microsporidia suppresses apoptosis in bmn. They are abundant in many ascomycetes and basidiomycetes and have also been found in microsporidia nosema bombycis and spraguea lophii, glomeromycetes glomus intraradices and several species of gigaspora, chytrids orpinomyces sp.
Pdf a monoclonal antibody to nosema bombycis and its use. On the function of the polar filament of nosema bombycis. Dnas from the reference strains of a microsporidium, nosema bombycis, were specifically amplified by polymerase chain reaction pcr using primers from a putative pseudogene together with those derived from conserved regions of a small subunit rrna gene of n. The history of sericulture speaks volumes of devastating outbreak of microsporidian disease in several european countries in the past tatsuke, 1971.
Glycoproteins play an glycoproteins play an important role in the process of adhesion and recognition, but the present results. Morphology and transcriptome analysis of nosema bombycis. For the control of chitin test, i have prepared chitin from the wings of honey bees. Pdf the classification of lower organisms abraham hernandez. Subcellular localisation of nbhxk2 protein in nosema bombycis bmn cells were inoculated with and grown on coverslips for 24 h. Nosema bombycis is a unicellular eukaryotic pathogen of the silkworm. Identification and subcellular localisation of hexokinase2. Antheraea pernyi, in the cell line of antheraea eucalypti were investigated, compared with those of nosema bombycis isolated from the silkworm, bombyx mori. The scfvg4, which we prepared from a mouse g4 monoclonal antibody, can target the n. Invitro cultivation of nosema bombycis sporoplasms. Field and laboratory studies on nosema bombycis infecting diamondback moth dbm, plutella xylostella l. Nosema bombycis is a destructive, obligate intracellular parasite of the bombyx. Research article open access comparative genomics of. This continuous domesticated made it susceptible to the attack of a number of pathogens.
Journal of invertebrate pathology 56, 395400 1990 a monoclonal antibody to nosema bombycis and its use for identification of microsporidian spores zhaoxi ke, weidong xie, xunzhang wang, qingxing long, and zhelong pu institute of entomology, zhongshan sun yatsen university, guangzhou, 510275, peoples republic of china received november 27, 1989. Jul 01, 2020 guo et al 2016 successfully transfected a gene for green fluorescence protein into some nosema bombycis cells using a nontransposon vector, illustrating that an exogenous gene can be integrated into the n. The tc1mariner elements belong to important component of dna transposon. Nosema bombycis, a silkworm bombyx mori parasite, was first described in 1857. Nov 26, 2010 a study on the prevalence of nosema bombycis infection in field populations of diamondback moth, plutella xylostella l. Engineered resistance to nosema bombycis by in vitro.
Nosema bombycis, diamondback moth, diadegma semiclausum, antibiotic, temperature introduction diamondback moth dbm, plutella xylostella l. We characterized a complete sec61 complex in nosema bombycis, which has been shown to consist of sec61. Swp25, a novel protein associated with the nosema bombycis. Pdf the reduced genome of the parasitic microsporidian. Here, we report the identification of the third spore wall protein of n. By comparing their genomes with a published distantly related nosema genome, n. This study was undertaken to examine the protein interactions of nosema. In nosema bombycis, two spore wall proteins have been characterizedan endosporal protein, swp30, and an exosporal protein, swp32. Easy labeling of proliferative phase and sporogonic phase. Most have been found in ascomycetes and basidiomycetes. Identification and subcellular localisation of hexokinase.
Ricinblectin enhances microsporidia nosema bombycis. Microsporidia can survive outside the host for several. Comparative genomics of parasitic silkworm microsporidia. Guo et al 2016 successfully transfected a gene for green fluorescence protein into some nosema bombycis cells using a nontransposon vector, illustrating that an exogenous gene can be integrated into the n.
Only a few have been identified in zygomycetes, glomeromycetes, chytrids, and microsporidia. Using the genome data of nosema bombycis, four families of miniature invertedrepeat transposable elements mites in ribosomal dna rdna were characterized in the microsporidian n. Nosema bombycis is a pathogen of silkworm pebrine that causes great economic. Interaction between swp9 and polar tube proteins of the. In malaysia, dbm was reported to cause serious damage on. In this study, the reactivity of the monoclonal antibody 2b10 was tested against an endospore protein of n.
Sep 24, 2004 preliminary genomic characterization of microsporidian nosema bombycis sironmani, t. Review history for a secretory hexokinase plays an active. Spore germination can be used for host cell invasion. Skip to main content accessibility help we use cookies to distinguish you from other users and to provide you with a better experience on our websites. In this study, we sequenced the genome of two microsporidian parasites. Pdf nbseptin2 expression pattern and its interaction with. The role of nbtmp1, a surface protein of sporoplasm, in. Nosema genomic dna moved as 23 kb fragment on a standard agarose gel.
Expression and localization of the spore wall protein swp26. Erjun ling, institute of plant physiology and ecology. Dried smears of spores of henneguya salminicola, nosema bombycis, and nosema. Specific amplification of dna from reference strains of. Feb 15, 2018 nosema bombycis is a destructive, obligate intracellular parasite of the bombyx mori. On complete solubilization, it showed peptide bands ranging from 17 to 68 kda.
Expression and localization of the spore wall protein. Dec 12, 2019 microsporidia nosema bombycis is the destructive pathogen in the production of sericulture. These results provide important insight into the biology and pathogenesis of microsporidia and potentially provide a possible strategy for genetic manipulation of microsporidia. Nbptp6 was rich in histidine h and serine s, which contained a signal peptide of 16 amino. Nosema bombycis, membrane protein, localization, rnai. Comparing the genomic regions that harbor the respective. Microsporidia are obligate intracellular parasites that infect a wide variety of host organisms, including humans. Identification and characterization a novel polar tube. Identification and subcellular localisation of hexokinase2 in.
An attempt was made to develop a new system for pebrine inspection. The microsporidian nosema bombycis is an obligate intracellular pathogen of the silkworm bombyx mori, causing the epidemic disease pebrine and extensive economic losses in sericulture. The name of the pathogen has been derived from two words that is nosema meaning disease and bombycis belongs to the scientific name of silkworm. It is the etiological agent of the deadly protozoan disease pebrine in the silkworm and inflicts severe worldwide economic losses in regions where sericulture is practiced, such as china, india, and other regions of the world 2. Nosema bombycis, which was first recognized in 1857 by nagely, led to the pebrine disease that had prevailed in europe, america, and asia during the mid19th century and which still causes heavy losses in silkproducing countries nagely, 1857. Germination, an indispensible process through which microsporidia infect the host cells, is regarded as a key developmental turning point for microsporidia from dormant state to reproduction state. Preliminary study on infect of nosema bombycis to pieris rapae. On the function of the polar filament of nosema bombycis volume 29 issue 2 skip to main content accessibility help we use cookies to distinguish you from other users and to provide you with a better experience on our websites. Oct 14, 2004 spore proteins of the microsporidian nosema bombycis, from the silkworm bombyx mori, were analysed by sdspolyacrylamide gel electrophoresis. Nosema bombycis, the first identified microsporidium, is a destructive pathogen of the silkworm bombyx mori and causes severe worldwide economic losses in sericulture. Major microsporidian structural proteins, such as the spore wall protein swp, are known to be involved in host invasion.
There was a significant difference in the percentage of nosema infection and mean infection intensity among diamondback moth larvae or pupae sampled from the cameron highlands and serdanggombak, each representative of high and. It was maintained at 26c in graces medi um supplemented with 50jo fetal bovine serum glb co and 50o of heat inactivated 60c for 15 min silkworm hemolymph. Indirect immunofluorescence assays and recombinant hk overexpressed in bmn. Quantitative proteomic analysis of germination of nosema. Latest status of silkwormassociated microsporidians via.
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